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ProCube brochure

Application note

11. ProCube Trial Service Case Study

For our most common ProCube Trial Service, we establish a protocol by expressing one protein from one construct. We take the customer’s plasmid and create a transfer vector, express protein in 5 silkworms and purify the protein using a single pass through an affinity purification column.

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10. Active Influenza Hemagglutinin

Vaccine discovery research is in need of large quantities of active membrane proteins. ProCube has developed the ProCube silkworm-baculovirus expression system (SBES) that couples the power of protein expression in a living bioreactor with the ease of scalability unsurpassed by conventional cell line expression systems.

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9. Case Study: Active Lactate Dehydrogenase (LD) Expressed and Purified

The function of a recombinant protein is dependent upon its structure. And providing the proper conditions is key for optimal protein expression of a functional protein.

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8. Production and Kinetic Analysis of Recombinant Antibodies

Although antibodies are commonly used in biopharmaceuticals, due to its complex structure including disulfide (S-S) bonds, it is known that its production efficiency from microorganisms is low.

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7. ProCube Trial Service Construct Survey

Expressing tag fusion proteins provides advantages such as improved detection, solubility and purification. Using the correct tag makes the difference between expressing a large amount of protein or expressing no protein at all.

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6. Expression Systems Production Efficiency Comparison (A Case Study Using Mouse IFNβ)

Many protein expression systems, such as E. coli, insect cell and mammalian cell lines, are available commercially and have their relative strengths and weaknesses. Among criteria for selecting an optimum expression system, high production efficiency is an important factor to consider when ordering proteins.

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5. Cysteine Protease Defective (CPd) Baculovirus Strain

One of the strengths of the ProCube silkworm-baculovirus expression system (SBES) is its capability to express a high quantity of target protein. However, we discovered that overexpressed target protein degrades within cells.

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4. Case Study: Expression of Human Chymase Enzyme

Human chymase is a serine protease found primarily in mast cells. It is an important drug target in cardiac therapy research. In order to mass-produce human chymase, we expressed recombinant chymase from our silkworm-baculovirus expression system (SBES), purified it, and evaluated its activity.

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3. Manufacturing IVD Reagent Component in Silkworm-Baculovirus Expression System with Minimal Lot-to-Lot Variability

Although we use naturally sourced materials, such as bovine cerebrum, for manufacturing some of our research use only or IVD reagents, as well as protein samples, they are known to lack lot stability and may carry risk of causing infectious diseases, such as bovine spongiform encephalopathy (BSE), in employees handling them.

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2. Active GPCR Expression and Purification

The field of drug discovery research is in need of large quantities of active membrane proteins. ProCube uses the silkworm-baculovirus expression system (SBES) that couples the power of protein expression in a living bioreactor with the ease of scalability unsurpassed by conventional cell line expression systems.

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1. Dock-Tagged Protein Purification System

Drug discovery demands highly efficient isolation and purification methods for tag fusion protein production. ProCube’s proprietary Dock-Tagged Protein Purification System delivers a highly versatile, extremely efficient expression and purification tag.

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